AP Lab 5 Cell Respiration
Lab #5: Cellular Respiration The purpose of this lab was to determine the rate of cellular respiration in . Describe and explain the relationship between. AP Biology lab 5 - Free download as Word Doc .doc), PDF File .pdf), Text File . txt) The rate of cellular respiration is higher in the germinating peas in cold than in the Describe and explain the relationship between the amount of oxygen. Lab 5 Cellular Respiration by Kris Layher Describe and explain the relationship between the amount of oxygen consumed and time. The amount of oxygen.
IF the temperature changes and the number of gas molecules is kept constant, then either pressure or volume or both will change in direct proportion to the temperature.
Carbon dioxide is removed so the change in the volume of gas in the respirometer will be directly proportional to the amount of oxygen that is consumed. In the experiment water will move toward the region of lower pressure.
lab 5 ap sample4 -
During respiration, oxygen will be consumed and its volume will be reduced to a solid. The result is a decrease in gas volume within the tube, and a related decrease in pressure in the tube.
The respirometer with just the glass beads will allow changes in volume due to changes in atmospheric pressure or temperature changes. The respirometer with only germinating peas will have a larger consumption of oxygen and will have a larger amount of CO2 that is converted into K2CO3 than the respirometer with beads and dry peas and the respirometer with beads alone.
Lab 5 Cellular Respiration by Kris Layher -
The materials used in the lab are as follows: First, set up both a room temperature 25oC and a 10oC water bath. Make sure you allow time to adjust the temperature in each bath. To obtain a temperature of 10oC add ice to of the baths until the temperature in the bath is 10oC.
Next, obtain a mL graduated cylinder and fill it with 50 mL of water. Drop in 25 germinating peas and determine the amount of water that is displaced.AP Biology Lab 5: Cellular Respiration
Record the volume of the 25 germinating peas. Then remove these peas and place them on a paper towel. They will be used in respirometer 1. Next, refill the graduated cylinder with 50 mL of water and drop 25 non-germinating peas into it.
Gently push the sensor down into the bottle until it stops. The sensor is designed to seal the bottle without the need for unnecessary force. Wait two minutes, then begin collecting data. Record the readings for oxygen gas in your data table every minute for 10 minutes.
When data collection has finished, remove the O2 Gas Sensor from the respiration chamber. Place the peas in a mL beaker filled with cold water and an ice cube. Fill the respiration chamber with water and then empty it. Thoroughly dry the inside of the respiration chamber with a paper towel. Obtain 25 non-germinating peas and place them in the respiration chamber 9.
Repeat Steps 4—7 for the non-germinating peas. Respiration of germinating peas in different temperatures Remove the germinating peas from the cold water and blot them dry between two paper towels. Repeat Steps 3—7 to collect data with the germinating peas at a cold temperature. Make a graph showing the results of the experiment.
lab 5 ap sample4
All data collected should be displayed on one graph. Be sure to clearly label both axes don't forget unitsused different colors and give a key as needed, use appropriate scaling on both axes, and provide an appropriate title.
Describe the relationship between the amount of oxygen consumed and time. What is the effect of germination on the rate of cell respiration in peas? What is the effect of temperature on the rate of cell respiration in peas?
Add ice to room temperature water and watch the thermometer until the temperature has reached degrees Celsius. For respirometer one, obtain a graduated cylinder and fill it with 50mL of water.
Drop in 25 germinating peas and determine the water displacement. Record the volume, remove the peas and place them on a paper towel. For respirometer two, obtain the same graduated cylinder, filled again with 50mL of water.
lab 5 Ap sample 2 cell resp
Drop twenty-five of the non-germinating peas in the water and continue adding beads to the water until the same water displacement for the non-germinating peas equals the first result. Remove the contents, and drain the water leaving the peas and beads to dry on a paper towel. For respirometer three, fill the mL graduated cylinder with 50mL of water and obtain the first water displacement value by adding just beads to the water in the cylinder. Take out the beads, allow the water to drain, and repeat this same procedure for respirometers 4, 5, and 6, which will be placed in the cooler water.
For assembly of the respirometers, obtain 6 vials, each with a stopper and a pipette. Make sure the vials are dry on the inside.
Do not get KOH on the sides of the respirometer. Place a small wad of non-absorbent cotton on top of the KOH saturated cotton, making sure the same amount is used for each respirometer. Place the first set of peas in their respective vials.
Do the same for the second set of peas.